| 摘要 |
Genomic Signature Tags (GSTs) are the products of a method for identifying and quantitatively analyzing genomic DNAs. The DNA is initially fragmented with a type II restriction enzyme. An oligonucleotide adapter containing a recognition site for MmeI, a type IIS restriction enzyme, is then used to release 21 bp tags from fixed positions in the DNA relative to the sites recognized by the fragmenting enzyme. These tags are PCR-amplified, purified, concatenated into longer molecules, and then cloned and sequenced. The tag sequences and abundances are used to create a GST profile that can identify and quantify the genome of origin within any complex DNA isolate. The total number of GSTs generated from a sample is determined by the incidence of recognition sites for the initial fragmenting enzyme.
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