Compositions and methods for agrobacterium transformation of plants

摘要

The present invention is directed to variants of Agrobacterium tumefaciens. These variants are either resistant to the effects of MDIBOA/DIMBOA, or hypersensitive to phenolic induction. These variants are improved over wild-type Agrobacterium in their ability to transform plant cells. Also provided are methods for their selection. In a distinct embodiment, there also is provided a modified Ti plasmid that increases the ability of an Agrobacterium strain to transform host cells. The plasmid contains virA and virG genes, under the control of the coliphage T5 PN25 promoter.