发明名称 |
Process for the production of alanine by recombinant microorganisms |
摘要 |
A process for preparing alanine by culturing a genetically modified microorganism that produces alanine from glucose. The microorganism is one that has been modified to result in an overexpression of an endogenous alanine dehydrogenase gene, expression of a heterologous alanine dehydrogenase gene, or a combination of both. The source of alanine dehydrogenase may be Bacillus sphaericus. The modified microorganisms may be a homofermentative microorganism, such as a strain of L. lactis. The process can be used for in situ and stereoscopic preparation of alanine. The invention also relates to the microorganisms and the recombinant DNA molecules that can be used in the invention, as well as to alanine and alanine-containing products obtained according to the process of the invention.
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申请公布号 |
US6627420(B1) |
申请公布日期 |
2003.09.30 |
申请号 |
US20000600449 |
申请日期 |
2000.11.30 |
申请人 |
NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK |
发明人 |
HOLS PASCAL;KLEEREBEZEM MICHIEL;KUIPERS OSCAR PAUL;FERAIN THIERRY;DELCOUR JEAN MARCELIN ALAIN MARIE GHISLAIN;DE VOS WILLEM MEINDERT |
分类号 |
A23C19/032;C12N1/21;C12N9/06;C12N15/09;C12P13/06;C12R1/01;C12R1/225;(IPC1-7):C12P13/06 |
主分类号 |
A23C19/032 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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