SELECTION OF PCR PRIMER PAIRS TO AMPLIFY A GROUP OF NUCLEOTIDE SEQUENCES

摘要

The present invention provides a method of determining a set of primer pairs for amplifying a group of related nucleotide sequences. A method of the invention is performed by identifying a group of related nucleotide sequences; generating the set of primers that matches each of the related nucleotide sequences; determining for each systematic pairing of each primer which of the related nucleotide sequences are amplified; and selecting from the systematic pairings a subset which amplifies all of the related nucleotide sequences. The invention also provides a method of using a set of primer pairs, which amplify a group of related nucleotide sequences, to identify nucleotide sequences related to the original group of nucleotide sequences. The invention also provides a computer apparatus for carrying out the computer-executed steps of the method. The invention further provides a computer program product comprising a signal bearing media for carrying out the method.