发明名称 |
METHOD FOR DETECTING CYTOSINE METHYLATION PATTERNS HAVING HIGH SENSITIVITY |
摘要 |
The invention relates to a method for detecting cytosine methylation in DNA samples. The inventive method comprises the following steps: a genomic DNA sample containing DNA to be examined and background DNA is chemically treated in such a way that all non-methylated cytosine bases are converted into uracil, the 5-methylcytosine bases remaining unchanged; the chemically treated DNA samples are amplified using at least two primer oligonucleotides and one polymerase, the DNA to be examined being preferred as a template as opposed to the background DNA; the amplificates are analysed; and the methylation status in the DNA to be examined is inferred from the presence of an amplificate and/or from the analysis of further positions. |
申请公布号 |
WO02072880(A3) |
申请公布日期 |
2003.09.25 |
申请号 |
WO2002EP02572 |
申请日期 |
2002.03.08 |
申请人 |
EPIGENOMICS AG;OLEK, ALEXANDER;BERLIN, KURT |
发明人 |
OLEK, ALEXANDER;BERLIN, KURT |
分类号 |
G01N27/62;C12N15/09;C12Q1/02;C12Q1/68;G01N21/78;G01N27/447;G01N30/88;G01N33/483;G01N33/50;G01N33/58 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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