SEROLOGICAL METHOD FOR DETECTING INTESTINAL MICROVILLI ATROPHY IN PATIENTS SUFFERING FROM COELIAC DISEASE

摘要

Method for detecting intestinal microvilli atrophy in a patient suffering from coeliac disease including immunological detection of anti F-actin autoantibodies in a biological fluid from the patient consisting of the following steps: a) preparation of a normal epithelial cell culture from small bowel of experimental animal; b) incubation with the patient fluid; c) detection of the binding of anti F-actin autoantibodies to the cells. Further said method can include, following step a) the incubation with a substance able to induce the polymerisation of globular actin to filaments.