发明名称 |
INTACT MINICELLS AS VECTORS FOR DNA TRANSFER AND GENE THERAPY IN VITRO AND IN VIVO |
摘要 |
A composition comprising recombinant, intact minicells that contain a therapeutic nucleic acid molecule is disclosed. Methods for purifying a preparation of such minicells also are disclosed. Additionally, a genetic transformation method is disclosed, which comprises (i) making recombinant, intact minicells available that contain a plasmid comprised of a first nucleic acid segment, and (ii) bringing the minicells into contact with mammalian cells that are engulfing-competent, such that the minicells are engulfed by the mammalian cells, which thereafter produce an expression product of the first nucleic acid segment. |
申请公布号 |
WO03033519(A3) |
申请公布日期 |
2003.09.25 |
申请号 |
WO2002IB04632 |
申请日期 |
2002.10.15 |
申请人 |
BRAHMBHATT, HIMANSHU;MACDIARMID, JENNIFER |
发明人 |
BRAHMBHATT, HIMANSHU;MACDIARMID, JENNIFER |
分类号 |
C12N15/09;A61K38/16;A61K48/00;C12N1/21;C12N5/10;C12N15/87 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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