| 摘要 |
It is disclosed herein a mass spectrometry-based method aiming at the separation and identification of naturally occurring peptides (self-existing molecules) and small molecular weight proteins NOP/SP of the solid biological materials. The separation of the protein molecules with weight less than 10kDa is obtained by mixing and homogenisation of the solid biological material with aqueous acetonitrile solution 50 per cent containing also 0,1per cent trifluoroacetic acid. Compared to the existingmethods in use, the present method dispenses with particularly complicated and time-consuming process phases (fractalisation, chromatography, hyper-filtering etc). The analysis of the NOP/SP by MALDI-TOF mass spectrometry makes said method be rapid and susceptible of automation and quantification what leads to a high-throughput method suitable for the diagnosis of pathologic situations either through analysis of one or several NOP/SP as specialized biological indices, or through comparison of physiological and pathogenic protein patterns profiles. |
