METHOD FOR MEASURING CHOLESTEROL IN HIGH-DENSITY LIPOPROTEIN, AND REAGENT FOR USE IN SAID METHOD

摘要

To provide a method for measuring HDL-C capable of enhancing the consistency with the measured value in accordance with a standard measuring method such as a DCM, even in a case where a specimen such as dyslipidemia exhibiting a special disease state is used as a measurement sample. HDL-C is measured by reacting a measurement sample, and cholesterol ester hydrolase and cholesterol oxidase, or cholesterol ester hydrolase and cholesterol dehydrogenase, in the presence of a compound represented by following general formula (I):;;wherein R1 represents a linear or branched alkyl group having 8 to 22 carbon atoms or a linear or branched alkenyl group having 8 to 22 carbon atoms, R2, R3 and R4 are the same as or different from each other and represent a linear or branched alkyl group having 1 to 6 carbon atoms or a linear or branched alkenyl group having 2 to 6 carbon atoms, and X− represents an anion, and a polyanion.

主权项

1: A method for measuring high-density lipoprotein cholesterol, the method comprising
reacting a measurement sample and cholesterol ester hydrolase and cholesterol oxidase, or cholesterol ester hydrolase and cholesterol dehydrogenase, in the presence of a polyanion and a compound represented by formula (I): wherein R1 represents a linear or branched alkyl group having 8 to 22 carbon atoms or a linear or branched alkenyl group having 8 to 22 carbon atoms, R2, R3 and R4 are each independently represent a linear or branched alkyl group having 1 to 6 carbon atoms or a linear or branched alkenyl group having 2 to 6 carbon atoms, and X− represents an anion.