| 摘要 |
Antibodies (Ak), in particular autoantibodies, such as autoantibodies against thyroid peroxidase (TPO), are determined by a procedure in which the sample to be investigated is reacted with two further, in particular monoclonal antibodies and an antigen. The two further antibodies are an immobilised antibody (Ak imm) and a freely labelled antibody (Ak*) against the antigen (Ag), and the latter is preferably used in the form of a crude, natural antigen, for example in the form of an organ extract. The presence of the antibodies (Ak) to be determined is evident as a disturbance of the formation of a sandwich of the two added antibodies (Ak imm and Ak*) with the added antigen (Ag) and as a consequent reduction in the binding of the label to the solid phase.
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