Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis

摘要

Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase.

主权项

1. A recombinant yeast host cell which produces isobutanol and comprises mitochondria which is substantially devoid of isopropylmalate synthase activity.