Absorbance spectrum scanning flow cytometry

摘要

The present invention provides systems and methods for analyzing the excitation spectra of fluorescent particles in a flowing stream. The system uses a white light laser and color separation optics to provide a spatially-distributed, continuous color-spectrum excitation light system that is used to illuminate a region of a flowing stream. A particle that passes through the detection region traverses the full dispersed spectrum of excitation light, and the fluorescence emissions from the particle are continuously measured as it passes through the detection region. The measured fluorescence emissions at each wavelength of excitation light, which changes through full spectrum of the excitation light as the particle passes through the detection region, provides the excitation spectrum of the particle.

主权项

1. A flow-type particle analyzer, the flow-type particle analyzer comprising:
a) an excitation light source that emits a collimated broad-spectrum excitation light beam, b) a cuvette channel, c) a dispersion element positioned between the excitation light source and the cuvette channel that disperses the spectrum of the excitation light beam to produce a continuous spectrum of continuously varying wavelengths of the excitation light beam on said cuvette channel such that a fluoresecent particle passing through a detection region of said cuvette channel in a fluid stream will traverse the continuous spectrum of continuously varying wavelengths of the excitation light beam; and d) detection optics that measure light emitted from fluorescent particles passing through the continuous spectrum of continuously varying wavelengths in the detection region of said cuvette channel.