MICROORGANISMS AS CARRIERS OF NUCLEOTIDE SEQUENCES CODING FOR CELL ANTIGENS USED FOR THE TREATMENT OF TUMORS

摘要

The invention relates to a microorganism with a nucleotide sequence coding f or a cell antigen in which the following components are inserted and are expressible: I) a nucleotide sequence coding for at least one epitope of an antigen of a tumor cell and/or a nucleotide sequence for at least one epitop e of an antigen that is specific for a tissue cell from which the tumor originates; II) an optional nucleotide sequence coding for a protein that stimulates cells of the immune system; IIIA) a nucleotide sequence for a transport system which makes it possible to express the expression product o f components I) and, optionally, II) on the outer surface of the bacterium and/or secrete the expression product of component I) and, optionally, of component II); and/or IIIB) a nucleotide sequence for a protein used for lysing the microorganisms in the cytosol of mammalian cells and for intracellularly releasing plasmids which are contained in the lysed microorganisms; and IV) an activation sequence for expressing one or several of components I) to IIIB), said activation sequence being selected among the group consisting of an activation sequence which is capable of being activat ed in the microorganism, is tissue-cell-specific but not cell-specific . Each o f components I) to IV) can be identically or differently arranged in an individual or multiple manner. Also disclosed are uses of such a microorgani sm for the production of a medicament.