| 摘要 |
PURPOSE: A recombinant bacterial system for producing MCL-PHA is provided, thereby mass-producing medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA). CONSTITUTION: A recombinant expression vector containing PHA synthesizing gene phaC, gene fadF, and/or gene fadD is provided, wherein the recombinant vector is p10499613C2. A transformant transformed with the recombinant expression vector p10499613C2 is provided, wherein the transformant is Escherichia coli WA101(p10499613C2), Escherichia coli WB101(p10499613C2), Escherichia coli WAB101(p10499613C2), Escherichia coli KA101(p10499613C2), Escherichia coli KB101(p10499613C2) or Escherichia coli KAB101(p10499613C2). A method for producing MCL-PHA comprises culturing a transformant selected from Escherichia coli WA101(p10499613C2), Escherichia coli WB101(p10499613C2), Escherichia coli WAB101(p10499613C2), Escherichia coli KA101(p10499613C2), Escherichia coli KB101(p10499613C2) or Escherichia coli KAB101(p10499613C2) in a medium containing fatty acid.
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