Method and device for selecting accelerated proliferation of living cells in suspension

摘要

A method and a device for selecting accelerated proliferation of living cells in suspension. The inventive culture apparatus (2) enables cells to proliferate in suspension over unlimited periods. Natural selection results in the accumulation of genetic variants which are increasingly better adapted to the chosen culture conditions. The organisms used can be prokaryotic or eukaryotic. The organisms used can be naturally occurring organisms or genetically modified. The inventive culture apparatus is also suitable for using constant, periodical or conditional culture conditions. The physical and chemical characteristics of the culture media used can be chosen by the user. The requirement that a population of cells proliferate exclusively in suspension in continuous culture conditions is satisfied by the periodical transfer of the organism suspension from a first culture vessel to a second culture vessel. After the transfer, the first culture vessel is subjected to a sterilising treatment and the sterilising agent is optionally neutralised, so that the first culture vessel is ready for the culture to be transferred back from the second culture vessel. The second culture vessel is then sterilised and neutralised.