| 摘要 |
<p>Gene expression doses expressed in fluorometric data, which are measured by an experiment with the use of DNA microarrays and DNA chips, of a comparative group and a control group are corrected based on a novel mathematic model. Based on the scatter plots thus corrected, a novel X-Y axis system having an x axis proprotional to the fluorescence intensity of genes is constructed. Next, windows each having a definite number of genes are made along the X-axis and the reliability limit of the arbitrary risk is determined in each window in accordance with Student’s t-distribution. Then windows are shifted by a definite number of genes in the X-axis direction and each reliability limit is determined. The plural reliability limits thus determined are complemented by smoothening (spline curve) to give a reliability curve of expression variation. Subsequently, genes located outside the reliability curve of expression variation thus obtained are extracted as variation genes.</p> |
