发明名称 |
Dna joining method |
摘要 |
The present invention provides a method to directionally clone any linear template DNA molecule into any linearized vector. The vector ends may be generated from any restriction enzyme cleavage. The method does not require a ligation step nor the use of carefully controlled conditions as is required with methods involving specific exonucleases alone. It has been determined that specific DNA polymerases are able to efficiently join one or more linear DNA molecules sharing ends with appropriate complementation.
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申请公布号 |
US2003162265(A1) |
申请公布日期 |
2003.08.28 |
申请号 |
US20030220262 |
申请日期 |
2003.01.08 |
申请人 |
EVANS DAVID H;WILLER DAVID O;YAO XIAO-DAN |
发明人 |
EVANS DAVID H;WILLER DAVID O;YAO XIAO-DAN |
分类号 |
C12N15/09;C12N15/66;(IPC1-7):C12Q1/68;C12P19/34;C12N15/85 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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