Cold-shock regulatory elements, constructs thereof, and methods of use

摘要

A fourth cold shock protein of the Escherichia coli CspA family is disclosed, as are the regulatory elements of the 5' UTR of the corresponding gene. The cspI gene is located at 35.2 min on the E. coli chromosome map, and CspI shows 70, 70, and 79% identity of CspA, CspB, and CspG, respectively. The 5'-untranslated region of the cspI mRNA consists of 145 bases and causes a negative effect on cspI expression at 37° C. The cspI mRNA was very unstable at 37° C. but was stabilized upon cold shock. The 5' UTR of cspI can enhance the translation of cold shock inducible genes under conditions that elicit a cold shock response in bacteria.