IMMUNOASSAY METHOD FOR MEMBRANE-BOUND MATRIX METALLOPROTEASE

摘要

It is made possible to quantify MT1-MMP and to measure the enzymatic activity of MT1-MMP sensitively, accurately and quickly with simple operations and reagents. The MT1-MMP quantifications and the MT1-MMP enzymatic activity measurements can be carried out using quantitative immunoassays for MT1-MMP with anti-MT1-MMP Ab and reagents used therefor, further immunologically quantifying methods for a member selected from (i) MT1-MMP released and/or solubilized from cell membrane with a surfactant and/or a reducing agent and (ii) spontaneously solubilized MT1-MMP, reagents used therefor, solid-phased MT1-MMP, etc., thereby enabling the screening for a compound which promotes or inhibits the expression of MT1-MMP or a compound which promotes or inhibits the enzymatic activity of MT1-MMP, and facilitating the development and research for valuable pharmaceutical drugs. There are also provided a useful testing drug for cancer or cancer metastasis.