| 摘要 |
DNA sequences encoding endothelial cell-leukocyte adhesion molecules ELAMs, methods for producing such molecules, and ELAMs (including the specific molecules ELAM1 and VCAM1 and 1b) essentially free of normally associated animal proteins are disclosed. Antibodies against ELAMs are also disclosed. DNA sequences encoding molecules involved in leukocyte adhesion (MILAs), methods for producing such molecules and MILAs (including the specific molecule, CDX) essentially free of normally associated animal proteins are also disclosed. Antibody preparations which are reactive for MILAs and also disclosed. We disclose DNA sequences designated clone 7.2 and clone 1, which cause cells transformed with them to express 1,3-fucosyl transferases and which are involved in CDX expression. We also disclose protein 7.2 and protein 1 which are encoded by clone 7.2 and clone 1, respectively. We also disclose Pseudo-X and Pseudo-X2, proteins which cause COS cells and CHO cells to bind to ELAM1 and to be recognized by alpha-CDX antibodies. Methods for identifying molecules which inhibit binding of leukocytes to endothelial cells, methods for inhibiting leukocyte binding to endothelial cells, and methods for detecting acute inflammation are disclosed.
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