发明名称 |
Universal probes and methods for detection of nucleic acids |
摘要 |
Signal primers are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The signal primer comprises a first and a second oligonucleotide and is partially single-stranded and partially double-stranded. In the presence of target, the second oligonucleotide of the signal primer is displaced from the first and a conformational change in a reporter probe occurs which changes the distance between the members of a donor/quencher dye pair linked to the reporter probe. The change in proximity between the dyes causes an increase or a decrease in fluorescence quenching, which is detected as an indication of the presence of the target sequence.
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申请公布号 |
US2003148303(A1) |
申请公布日期 |
2003.08.07 |
申请号 |
US20020163862 |
申请日期 |
2002.06.05 |
申请人 |
NADEAU JAMES G.;LINN C. PRESTON;PITNER J. BRUCE;DEAN CHERYL H.;WALKER G. TERRANCE |
发明人 |
NADEAU JAMES G.;LINN C. PRESTON;PITNER J. BRUCE;DEAN CHERYL H.;WALKER G. TERRANCE |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
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