发明名称 |
Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
摘要 |
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
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申请公布号 |
US6602986(B1) |
申请公布日期 |
2003.08.05 |
申请号 |
US20000713920 |
申请日期 |
2000.11.15 |
申请人 |
MAXYGEN, INC. |
发明人 |
STEMMER WILLEM P. C.;CRAMERI ANDREAS |
分类号 |
C12N15/09;A61K38/00;A61K39/395;C07K14/435;C07K14/545;C07K16/00;C12N9/38;C12N9/86;C12N15/00;C12N15/10;C12P21/08;C12Q1/68;C40B40/02;G01N33/53;G01N33/566;(IPC1-7):C12P21/08 |
主分类号 |
C12N15/09 |
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