发明名称 |
Method and device for integrated protein expression, purification and detection |
摘要 |
A method for presenting a target protein or an array of target proteins for analysis is disclosed. The method includes the steps of forming a coding sequence; placing the coding sequence and protein-synthesis components capable of expressing the target protein under selected protein-synthesis conditions in a well on a substrate, the well having a surface which has been functionalized with the second coil-forming peptide; expressing said coding sequence under such conditions, wherein the target protein so synthesized binds to the well through coil-coil heterodimer formation, and is thus presented for analysis in the well in captured form; and washing said well to remove unbound components. The coding sequence includes a first nucleic acid sequence which encodes a first coil-forming peptide having a selected charge and being capable of interacting with a second, oppositely charged coil-forming peptide to form a stable alpha-helical coiled-coil heterodimer; and a second nucleic acid sequence encoding the target protein. Also disclosed are kits for use in practicing the method.
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申请公布号 |
US2003143576(A1) |
申请公布日期 |
2003.07.31 |
申请号 |
US20020225788 |
申请日期 |
2002.08.22 |
申请人 |
CHAO HEMAN;WONG WAH Y.;TIAN BAOMIN;SEGAL DONALD;MCELROY JERRY |
发明人 |
CHAO HEMAN;WONG WAH Y.;TIAN BAOMIN;SEGAL DONALD;MCELROY JERRY |
分类号 |
G01N33/53;C12N15/09;C12P21/02;C40B30/04;C40B40/10;G01N33/543;G01N33/566;G01N33/68;G01N37/00;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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