发明名称 |
Detection of antibodies to bacterial antigens by fluorescence polarization |
摘要 |
In a homogeneous immunoassay, fluorophore-conjugated lipopolysaccharide derived bacterial antigens are reacted with antibodies specific for the antigens. Quantitative detection of the formation of an immune complex is obtained by measuring the change in fluorescence polarization after complex formation. The reaction occurs quickly (less than two minutes to equilibrium), and involves the addition of only one reagent to a diluted serum specimen. The absence of a solid phase separation step eliminates false positive results and increases throughput.
|
申请公布号 |
US6596546(B1) |
申请公布日期 |
2003.07.22 |
申请号 |
US19990400798 |
申请日期 |
1999.09.22 |
申请人 |
JOLLEY MICHAEL E.;NIELSEN KLAUS H. |
发明人 |
JOLLEY MICHAEL E.;NIELSEN KLAUS H. |
分类号 |
G01N33/569;(IPC1-7):G01N33/533;C07G17/00;C07H1/00 |
主分类号 |
G01N33/569 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|