METHOD OF ASSAY OF ABILITY OF TESTING SAMPLE TO EFFECT ON INTERACTION OF BINDING FIRST AND SECOND PEPTIDE IN PAIR OF PEPTIDE BINDING (VARIANTS) AND METHOD OF ASSAY OF BINDING ORDER OF TESTING SAMPLE WITH PEPTIDE IN PAIR OF PEPTIDE BINDING (VARIANTS)

摘要

FIELD: molecular genetics and biology. SUBSTANCE: invention involves carrying out the assay of activity of gene-reporter in addition of testing sample to modified cell of the host under conditions useful for detection of this activity in the presence of sample showing the binding interaction in pair of peptide binding. Activity of gene-reporter is determined by measurement of selected phenotype that depends on activity of reporter. Observation of alternation in measuring phenotype of modified cell of the host is carried out being in this cell gene-reporter is effectively associated with yeast protein of transcription activation in addition of testing sample to the host cell. Activity of gene-reporter is determined by measurement of selected phenotype that depends on activity of reporter. The host cell can display alternation in phenotype in the presence of testing sample only that binds only with a single peptide of pair of peptide binding. The level of activity of gene-reporter of the modified host cell is determined in addition of testing sample to mentioned cell under conditions suitable for detection of mentioned activity. Invention allows the discovering compounds that can interact as ligands for specific receptors or tranductors. EFFECT: improved assay method, valuable biological properties of sample. 58 cl, 18 dwg, 1 tbl, 11 ex