| 摘要 |
<p> ABSTRACT The present invention concerns a method for preparing liposomes containing a at least one biologically active substance encapsulated therein, said method comprising (I) (A) (a) dissolving at least one liposome-forming lipid and the at least one biologically active substance in a water-miscible organic solvent to form a mixture; (b) (i) dissolving at least one liposome-forming lipid in a water-miscible organic solvent to form an organic solution; (ii) dissolving the at least one biologically active substance in aqueous medium X to form an aqueous solution; and (iii) mixing the organic solution and aqueous solution to form a mixture; or (c) mixing at least one liposome-forming lipid, the at least one biologically active substance and a water-miscible organic solvent to form a mixture; or (B) mixing at least one liposome-forming lipid and a water- miscible organic solvent to form a mixture; (II) (A) mixing the mixture of step (I)(A) with aqueous medium Y and optionally the at least one biologically active substance to form a gel or liquid containing gel particles; or (B) mixing the mixture of step (I)(B) with the at least one biologically active substance and aqueous medium Y to form a gel or liquid containing gel particles; and thereafter (III) (A) mixing the gel or liquid containing gel particles with aqueous medium Z1 to directly form the liposomes containing the at least one biologically active substance encapsulated in the liposomes; or (B) (i) mixing the gel or liquid containing gel particles with aqueous medium Z1 to form a waxy substance; and (ii) mixing the waxy substance with aqueous medium Z2 to directly form the liposomes containing the at least one biologically active substance encapsulated in the liposomes; wherein aqueous media X, Y, Z1 and Z2 are the same or different, and a phospholipid content of the gel or the liquid containing gel particles in step (II) is not 15 to 30% by weight of the gel or liquid containing the gel particles.</p> |
