METHOD FOR PREPARING TRANSFORMED CUCUMIS SATIVUS

摘要

The present invention relates to a method of preparing a transformed Cucumis sativus using Acrobacterium tumefaciens, more particularly, to a method for preparing a transformed Cucumis sativus, which comprises the steps of: (a) inoculating a cotyledon from Cucumis sativus with Agrobacterium tumefaciens harboring a suitable vector; (b) placing the inoculated cotyledon on a medium containing BAP (6-benzylaminopurine) and NAA (a-naphthalene acetic acid) and culturing the inoculated cotyledon to obtain regenerated shoots; and (c) culturing the regenerated shoots on a rooting medium to obtain the transformed Cucumis sativus.