发明名称 |
High efficiency encapsulation of charged therapeutic agents in lipid vesicles |
摘要 |
Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50%.
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申请公布号 |
US2003129221(A1) |
申请公布日期 |
2003.07.10 |
申请号 |
US20010895480 |
申请日期 |
2001.06.29 |
申请人 |
SEMPLE SEAN C.;KLIMUK SANDRA K.;HARASYM TROY;HOPE MICHAEL J.;ANSELL STEVEN M.;CULLIS PIETER;SCHERRER PETER;DEBEYER DAN |
发明人 |
SEMPLE SEAN C.;KLIMUK SANDRA K.;HARASYM TROY;HOPE MICHAEL J.;ANSELL STEVEN M.;CULLIS PIETER;SCHERRER PETER;DEBEYER DAN |
分类号 |
A61K9/127;A61K9/50;A61K31/7105;A61K31/711;A61K47/28;A61K47/44;A61K48/00;(IPC1-7):A61K48/00 |
主分类号 |
A61K9/127 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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