发明名称 |
METHOD FOR THE IN VITRO SELECTION OF BINDERS |
摘要 |
The invention relates to a method for the in vitro selection of binders capable of binding to a target molecule, from a molecule library. According to the inventive method, immobilised target molecules are brought into contact with a plurality of molecules bound to a reference complex, so that target molecules can react with possible binders and form a linkage from the reference complex, binding agent and target molecule. Non-bound constituents can then be separated and a force is applied to the linkages such that a link in the linkage is dissolved, and the binders bound to the target molecules and or complementary binding agents thereof are identified and/or amplified. Said reference complex consists of two constituents, the binding force thereof being selected in such a way that, when a tractional force is applied, the binding force of the two reference constituents is smaller than the binding force of a specifically bound molecule. |
申请公布号 |
WO03056034(A2) |
申请公布日期 |
2003.07.10 |
申请号 |
WO2002EP14584 |
申请日期 |
2002.12.19 |
申请人 |
NANOTYPE GMBH;STEIPE, BORIS;CLAUSEN-SCHAUMANN, HAUKE;OESTERHELT, FILIPP |
发明人 |
STEIPE, BORIS;CLAUSEN-SCHAUMANN, HAUKE;OESTERHELT, FILIPP |
分类号 |
C12N15/11;C12N15/115;C12Q1/68;G01N33/543 |
主分类号 |
C12N15/11 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|