| 摘要 |
Synthetic nucleotide constructs for analysis of the human coagulation factor V gene, the human methylenetetrahydrofolate reductase gene, the human prothrombin gene, the human coagulation factor 8 gene and the human von Willebrand gene are disclosed wherein each of said constructs: - is capable of forming a duplex with a fragment of its respective gene, whether said gene does or does not carry a particular polymorphism, such that, upon formation of the duplex, the construct spans the point of the possible polymorphism. - has a nucleotide sequence of up to 500 base-pairs. - has an identifier within 100 nucleotides relative to the site of the possible polymorphism in the gene. Also disclosed are methods for examining said genes with said constructs, methods of forming said constructs, test-kits and a method of simultaneously examining a plurality of regions of genomic DNA. |
