发明名称 |
METHODS FOR PRODUCING HYALURONAN IN A RECOMBINANT HOST CELL |
摘要 |
The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase. |
申请公布号 |
CA2803931(A1) |
申请公布日期 |
2003.07.03 |
申请号 |
CA20022803931 |
申请日期 |
2002.12.20 |
申请人 |
NOVOZYMES BIOPHARMA DK A/S |
发明人 |
SLOMA, ALAN;BEHR, REGINE;WIDNER, WILLIAM;TANG, MARIA;STERNBERG, DAVID;BROWN, STEPHEN |
分类号 |
C12N15/09;C12P19/26;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/00;C12N9/04;C12N9/10;C12N9/16;C12N15/52;C12N15/53;C12N15/54;C12N15/61;C12N15/62;C12N15/63;C12P19/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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