METHOD OF IDENTIFYING GIBBERELLIN RESPONSIVE GENE USING CULTURED VEGETABLE CELLS

摘要

It has never been considered in the art that cultured cells response to gibberellin. To comprehensively and conveniently identify and isolate gibberellin responsive genes, however, cultured cells which are uniform and easy to handle are appropriate. Considering these advantages of cultured cells, attempts are made to develop a method of identifying and isolating gibberellin responsive genes with the use of cultured cells. Cultured cells are pre-cultured in an auxin-free medium. Then, it is examined whether or not changes in gene expression are detected. As a result, it is surprisingly found out that some genes show changes in the expression after the gibberellin treatment.