发明名称 METHOD FOR DETECTING MICROORGANISM
摘要 PROBLEM TO BE SOLVED: To provide a method for rapidly easily detecting microorganisms captured on a filtration film in high sensitivity by using an inexpensive apparatus. SOLUTION: This method for detecting the microorganisms on the filtration film comprises following steps: (1) a first step for bringing the microorganisms captured on the filtration film, or an extract of the microorganisms into contact with a bioluminescent reagent to cause bioluminescence on the filtration film; and (2) a second step for exposing a photographic film to the bioluminescence on the filtration film. Preferably, the second step is carried out by making the luminescent filtration film and the photographic film come closely into contact directly or through a transparent material. The bioluminescent reagent preferably contains luciferase, luciferin and magnesium ion, and the photographic film is preferably an instant film. COPYRIGHT: (C)2003,JPO
申请公布号 JP2003180395(A) 申请公布日期 2003.07.02
申请号 JP20020106000 申请日期 2002.04.09
申请人 KIKKOMAN CORP 发明人 SAKAKIBARA TATSUYA;SUZUKI SHIGEYA;NISHIMOTO KAZUNORI;IGARASHI TOSHINORI;HATSUTORI NORIAKI;HARADA YASUHIRO;MURAKAMI SEIJI
分类号 G01N21/77;C12Q1/04;G01N21/78;G01N33/18;(IPC1-7):C12Q1/04 主分类号 G01N21/77
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