METHODS AND KITS FOR DETECTING PROTEIN KINASES

摘要

The invention provides a method for measuring protein kinase activity, said method comprising: (a) providing a first solution comprising ATP and a protein kinase to be tested, and a second solution comprising ATP in the absence of said kinase to be tested; (b) adding a substrate capable of being phosphorylated by the protein kinase to be tested to the first and second solutions of step (a); (c) measuring the concentration of ATP and/or ADP, or the rate of change thereof with respect to time, in each of the reaction mixtures formed in step (b) using a bioluminescence reaction; and (d) using the information about the concentration of ATP and/or ADP to determine the activity of the protein kinase to be tested. A particular advantage of the methods and kits of the invention is that they provide a generic protein kinase activity test, that is, a test which is not specific for a single protein kinase or its substrate.