| 摘要 |
<p>The present invention provides for an improved method of preparing fetuin by using a chelating agent to remove inorganic ions , such as zinc , calcium, and barium, from fetuin and then, reloading the 'naked' fetuin with Zinc Acetate to form a product that is mainly Fetuin-Zinc. This improved method of preparing Fetuin-Zinc or supercharged zinc fetuin increases the effectiveness of inducing apoptosis in cancer cells by three to four times. A specific peptide fragment was then derived from this specially prepared zinc charged fetuin. This polypeptide strongly induced apoptosis in LNCaP (prostate cancer) and HT-29 (colon cancer) cells without affecting CCD 18 co (normal colon) cells, The in vitro tissue culture study demonstrated that the polypeptide is approximately ten times more potent than the parent molecule (full-length zinc charged fetuin) in inducing apoptosis.</p> |
