High throughput assay for N-sulfotransferase activity of glucosaminyl N-deacetylase/N-sulfotransferases

摘要

Assays for N-sulfotransferase activity of glucosaminyl N-deacetylase/N-sulfotransferases (NDSTs) include transferring a radiolabeled sulfate from a sulfate donor to a polysaccharide acceptor in the presence of an NDST enzyme to form a radiolabeled sulfated polysaccharide, the radiolabeled sulfated polysaccharide which when bound to a potentially scintillating particulate within an activating distance emits a detectable signal indicative of N-sulfotransferase activity. The assay provides high throughput assays for screening inhibitors that block N-sulfotransferase activity of an NDST. Specific compounds that inhibit N-sulfotransferase activity of an NDST and therapeutic uses of these compounds are disclosed.