| 摘要 |
Kit for detection and/or quantification of a ligand (C) using a receptor (A) comprises: <??>(a) a single- or double-stranded nucleotide sequence (B); and <??>(b) as (A), a monomeric or multimeric protein with at least one recognition site (RS1) specific for (C) and a second site (RS2) specific for (B), where (C) modulates, positively or negatively, binding of (A) through RS2, is new. <??>Independent claims are also included for: <??>(1) detection and/or quantification of (C) in a sample, preferably biological, by contacting the sample with the new kit and detecting/quantifying (C) by immunochromatography, enzyme measurements (particularly enzyme-linked immunosorbant assay), surface plasmon resonance (SPR) or mass spectrometry (MS); and <??>(2) detection and/or quantification of (C), at least partly in vitro, using many elements, preferably processes and/or substances known to be involved in the natural mechanisms of regulating gene expression in vivo, including the use of (A), originating from the natural mechanisms, that has several specific recognition sites, where the reactivity of each site with respect to its own ligand, can modify the reactivity of another site in (A).
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