发明名称 |
PROCESS FOR THE PRODUCTION OF POLYPEPTIDES IN MAMMALIAN CELL CULTURES |
摘要 |
The invention provides a method for producing a polypeptide of interest which method comprises: providing a transformed host cell which comprises a nucleotide sequence which encodes a polypeptide of interest and which directs expression of the polypeptide of interest in the transformed host cell, or a hybridoma which comprises a nucleotide sequence encoding a polypeptide of interest; providing a culture medium suitable for mammalian cell culture; and culturing the hybridoma or transformed host cell in the culture medium under conditions that allow for expression of the polypeptide of interest, wherein the culture medium (i) comprises water, a buffer, an energy source, amino acids, a lipid source or precursor, a source of iron, non-ferrous metal ions, inorganic salts, vitamins and cofactors; and (ii) is free from each of a plant-derived or animal-derived peptone, a functional protein, and a full-length polypeptide. The invention further relates to a transformed host cell and to suitable low-cost media applicable in the method of the present invention. |
申请公布号 |
WO03046162(A2) |
申请公布日期 |
2003.06.05 |
申请号 |
WO2002EP13431 |
申请日期 |
2002.11.28 |
申请人 |
POLYMUN SCIENTIFIC IMMUNBIOLOGISCHE FORSCHUNG GMBH;KATINGER, HERMANN;KUNERT, RENATE;MUELLER, DETHARDT;UNTERLUGGAUER, FLORIAN |
发明人 |
KATINGER, HERMANN;KUNERT, RENATE;MUELLER, DETHARDT;UNTERLUGGAUER, FLORIAN |
分类号 |
C07K14/505;C12N15/85;C12N15/90;C12P21/02;C12P21/08 |
主分类号 |
C07K14/505 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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