FARBKOMPENSATION VON ÄNDERUNGEN BEDINGT DURCH TEMPERATUR UND ELEKTRONISCHE PHÄNOMENE

摘要

The present invention is directed to a method of analyzing at least two analytes at the same time using at least two fluorescent detecting entities. More particularly, the present invention relates to the use of fluorescently labeled hybridization probes to identify the genotypes of a nucleic acid sample at more than one locus by measuring fluorescence throughout a range of temperatures and correcting for temperature-dependent spectral overlap of the fluorescent probes.