HYBRID OLIGONUCLEOTIDE PRIMERS FOR AMPLIFICATION OF DNA AND USES THEREOF

摘要

The present invention provides compositions and methods for the determination of the presence of specific microorganisms in a sample using the amplification of DNA. The invention further provides compositions and methods that provide an internal standard for the validation of the amplification of DNA. The invention is based on the use of hybrid oligonucleotides that have two domains that are specific for two genetically distinct regions of DNA, e.g., in two genetically distinct microorganisms.