FUNCTIONAL PROTEOMICS USING DOUBLE PHAGE DISPLAY SCREENING

摘要

The present invention relates to protein identification. In one aspect, the invention provides a method for the identification of a first protein or of a second protein containing an idiotypic region of the first protein comprising (a) contacting said first protein with an antibody phagemid library to form a complex between said first protein and at least one member of said library, (b) screening a cDNA phagemid library of the proteome of a tissue of interest with the complex forming antibody to identify a protein-specific phagemid that displays a protein that binds the antibody phagemidi, (c) identifying the second protein from the cDNA of the protein-specific phagemid. In a second aspect, the method involves as step (b) screening a ribosome display library of the proteome of the tissue of interest with the complex forming antibody obtained from step (a) to identify a protein-specific ribosome that dis plays a protein that binds the antibody phagemid. If the antibody phage yield is low, the phage DNA may be amplified prior to reinfecting bacteria.