发明名称 Differential PCR-RFLP assay for detecting and distinguishing between nonpathogenic PCV-1 and pathogenic PCV-2
摘要 The present invention relates to a method for detecting and differentiating PCV infections in a biological sample taken from a pig which involves amplifying a fragment from an extracted nucleic acid; digesting the fragment with a suitable restriction enzyme such as the unique NcoI restriction enzyme; forming a restriction fragment length polymorphism pattern; and then detecting the presence or absence of a PCV isolate. The invention further concerns the new oligonucleotide primers for differentiating PCV infections comprising a nucleotide sequence selected from the group consisting of MCV1 having a nucleotide sequence set forth in SEQ ID NO: 1 and MCV2 having a nucleotide sequence set forth in SEQ ID NO: 2. Moreover, this invention provides a novel kit for detecting and distinguishing PCV infections that includes the new oligonucleotide primers and the suitable restriction enzyme.
申请公布号 US2003096377(A1) 申请公布日期 2003.05.22
申请号 US20020184191 申请日期 2002.06.27
申请人 VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. 发明人 MENG XIANG-JIN;FENAUX MARTIJN
分类号 C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/70;C12P19/34 主分类号 C12Q1/68
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