发明名称 |
Differential PCR-RFLP assay for detecting and distinguishing between nonpathogenic PCV-1 and pathogenic PCV-2 |
摘要 |
The present invention relates to a method for detecting and differentiating PCV infections in a biological sample taken from a pig which involves amplifying a fragment from an extracted nucleic acid; digesting the fragment with a suitable restriction enzyme such as the unique NcoI restriction enzyme; forming a restriction fragment length polymorphism pattern; and then detecting the presence or absence of a PCV isolate. The invention further concerns the new oligonucleotide primers for differentiating PCV infections comprising a nucleotide sequence selected from the group consisting of MCV1 having a nucleotide sequence set forth in SEQ ID NO: 1 and MCV2 having a nucleotide sequence set forth in SEQ ID NO: 2. Moreover, this invention provides a novel kit for detecting and distinguishing PCV infections that includes the new oligonucleotide primers and the suitable restriction enzyme.
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申请公布号 |
US2003096377(A1) |
申请公布日期 |
2003.05.22 |
申请号 |
US20020184191 |
申请日期 |
2002.06.27 |
申请人 |
VIRGINIA TECH INTELLECTUAL PROPERTIES, INC. |
发明人 |
MENG XIANG-JIN;FENAUX MARTIJN |
分类号 |
C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/70;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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