A method for measuring intracellular ATP and/or gene expression, which method comprises transforming a cell with a construct which encodes a luciferase and a luciferin recycling protein, introducing luciferin into said cell, and monitoring biolominescent signals from said cell. Novel luciferin recycling proteins are also described and these, together with their use in the production of optically active enzyme substrates are claimed.
申请公布号
WO03042693(A2)
申请公布日期
2003.05.22
申请号
WO2002GB05120
申请日期
2002.11.13
申请人
THE SECRETARY OF STATE FOR DEFENCE;DAY, JOHN, CAVENDISH;SQUIRREL, DAVID, JAMES;BAILEY, MARK, JOHN;WHITE, PETER, JOHN
发明人
DAY, JOHN, CAVENDISH;SQUIRREL, DAVID, JAMES;BAILEY, MARK, JOHN;WHITE, PETER, JOHN