| 摘要 |
<p>Described is an improved method for introducing a site-directed mutation into a DNA sequence of interest in a target DNA using a single oligonucleotide mutagenic primer which is complementary to the DNA sequence to be mutated in the target DNA and wherein the mutation to be introduced into the DNA sequence by the primer is located within the region of complementarity. In a single reaction, the primer is annealed to the target DNA and extended in an extension/polymerization reaction to produce copies of the target DNA comprising the primer. A selection means is used to remove the target DNA and the copies of the target DNA comprising the primer are propagated in host cells. The method can be adapted to include a plurality of primers in the reaction which enables libraries of mutated DNAs to be produced. Also provided are kits for the improved site-directed mutagenesis method.</p> |
