| 摘要 |
<p>The invention relates to the field of diagnostics of nucleic acids, especial ly to a highly sensitive method for detecting, differentiating and characterizi ng nucleic acids in the form of a dry rapid test. The inventive dry rapid test contains a chromatographic material that comprises a sample reception zone, a separation zone including a binding area, in which one or more sequence- specific nucleic acid probes are immobilized, and a zone which absorbs a liquid down-stream of the separation zone including a binding area. Accordin g to the inventive method, the sequence-specific nucleic acid probes are immobilized via a polymer linker. The method comprises the following steps: i) denaturing, in the case of double-stranded nucleic acids, the nucleic acid t o be detected and then neutralizing it, ii) applying the nucleic acid to be detected to the sample reception zone in a run buffer which contains mildly denaturing agents, iii) the nucleic acid moving from the sample reception zo ne in direction of the liquid-absorbing zone, (iv) contacting the nucleic acid to be detected in the binding area of the separation path with the sequence- specific nucleic acid probe and hybridizing it with the sequence-specific nucleic acid probe, v) detecting the nucleic acid or the hybridization of th e nucleic acid with the sequence-specific nucleic acid probe via a label that is attached to the nucleic acid to be detected or via the detection of a label of the nucleic acid double strand. The invention further relates to a device fo r carrying out the method according to the invention.</p> |
