发明名称 |
In vivo electroporation method for early stage embryo of chickens |
摘要 |
A gene is transferred to the early stage chicken embryo by electroporation with placing the electrode tips to hold both ends of the embryo. Transfer of the desired gene to the early stage embryo was detected using the beta-galactosidase gene of E coli as a reporter gene. The expression of the gene in the early stage embryo of chicken was thus confirmed. This method for transferring a gene into undifferentiated cells is simple to implement, highly efficient in transferring a gene, and applicable to various animal species.
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申请公布号 |
US6563017(B2) |
申请公布日期 |
2003.05.13 |
申请号 |
US19990214565 |
申请日期 |
1999.09.13 |
申请人 |
DNAVEC RESEARCH INC. |
发明人 |
MURAMATSU TATSUO;SAKATA TSUNEAKI;HASEGAWA MAMORU |
分类号 |
A01K67/027;C12N15/85;C12N15/87;(IPC1-7):C12N15/87;C12N15/63;C12N15/00 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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