PROCESS FOR DETECTING SERINE/THREONINE KINASE ACTIVITY

摘要

Detection of threonine or serine kinase activity in an immunoassay involves incubating a labelled monoprephosphorylated protein orpeptide with a phosphate donor and a threonine or serine kinase to from bisphosphorylated protein or pepite and adding an antibody having a specificity to the bisphosphorylated form. The immunoassay is useful for detecting/screening modulators and inhibitors for serine or threonine kinase activity (claimed), and to screen compound libraries in order to locate molecules. The phosphorylation at a position of the substrate in a special proximity to an other site of phosphorylation not only enhances the affinity of the phosphorylated product to the detection antibody but also results in a high specificity for the other phosphorylated position and has a positive effect on the kinetic of the kinase reaction.