Modified oligonucleotides and uses thereof

摘要

Chimeric oligonucleotides are provided that contain non-modified DNA or RNA residues and modified nucleic acid residues. A modified nucleic acid residue is placed in the -1 position of the 3' and/or 5' end of the oligonucleotide. The oligonucleotides can exhibit significantly enhanced hybridization properties and improved capabilities as primers in nucleic acid extension and amplification reactions.