PRIMERS AND METHOD OF DETECTING BACTERIA

摘要

A first primer which can amplify in a detectable manner DNAs encoding the 16Sr RNAs of bacteria belonging to the genera of Escherichia, Salmonella and Vibrio when subjected to PCR under definite conditions, but does not amplify DNA encoding the 16Sr RNA of chloroplast and DNA encoding the 16Sr RNA of mitochondria when subjected to PCR under the same conditions; and a second primer which can amplify in a detectable manner DNAs encoding 16Sr RNAs of Staphylococcus aureus and Bacillus cereus when subjected to PCR under definite conditions, but does not amplify DNA encoding the 16Sr RNA of chloroplast and DNA encoding the 16Sr RNA of mitochondria when subjected to PCR under the same conditions.