FREE-FLOATING CRYOSTAT SECTIONS FOR IMMUNOELECTRON MICROSCOPY

摘要

A method is described of viewing a single tissue sample with a light microscope and an electron microscope. A tissue sample is cryopreserved. cryo sectioned and then floated in a cryoprotectant solution on a surface of a glass slide or in a well and is viewed by light microscopy. The step of floating the tissue sample does not destroy said tissue sample for subsequent electron microscope viewing. The same tissue sample in then prepared by conventional ultrastructural processing for viewing by electron microscopy. The data from the light microscope and electron microscope images of selected sites of the same tissue sample can be compared and analyzed.